Order ID:89JHGSJE83839 | Style:APA/MLA/Harvard/Chicago | Pages:5-10 |
Instructions:
Enzyme Kinetics: Extraction and Determination of
Wheat Germ Acid Phosphatase Activity
(Adapted from Modem Biology Inc.)
Background Information
Enzymes
Enzymes are proteins that function to speed up chemical processes in living cells. For
this reason they are often referred to as catalysts. There are numerous enzymes present in
all living cells. They serve to increase the velocity or speed of the chemical reactions that
take place in biological systems including breakdown, synthesis and chemical transfer
reactions. In doing this, enzymes keep biological systems functioning properly.
Figure 1-1. Enzyme Action.
Enzyme (E)
+
Substrate (S)
II
Enzyme-Substrate
Complex (E-S)
Enzyme (E)
+
Product (P)
Several components are involved in an enzymatic reaction. Substrate (S) is the
compound that is acted on by the enzyme. The enzyme binds to the substrate to form the
enzyme-substrate (ES) complex. This interaction is critical for the specific nature of
enzyme action. Why might formation of the ES complex be important for providing
specificity?
As illustrated in Figure 1-1 above, binding of the enzyme to the substrate has a lock and
key type interaction, meaning enzymes will only bind chemical species that fit the “lock”;
the specific nature of this interaction helps ensure that only the appropriate substrate is
acted on by the-enzyme. Once the ES complex is formed, the enzyme alters the structural
conformation of the substrate in such a way that the substrate is converted to a product.
The enzyme is simply used to facilitate the conversion of the substrate to product. Once
this occurs, the enzyme is released and free to combine with more substrate so that more
products can be generated.
Enzyme Kinetics Enzyme Kinetics: Extraction and Determination of Wheat Germ Acid Phosphatase Activity (Adapted from Modem Biology Inc.) Background Information Enzymes Enzymes are proteins that function to speed up chemical processes in living cells. For this reason they are often referred to as catalysts. There are numerous enzymes present in all living cells. They serve to increase the velocity or speed of the chemical reactions that take place in biological systems including breakdown, synthesis and chemical transfer reactions. In doing this, enzymes keep biological systems functioning properly. Figure 1-1. Enzyme Action. Enzyme (E) + Substrate (S) II Enzyme-Substrate Complex (E-S) Enzyme (E) + Product (P) Several components are involved in an enzymatic reaction. Substrate (S) is the compound that is acted on by the enzyme. The enzyme binds to the substrate to form the enzyme-substrate (ES) complex. This interaction is critical for the specific nature of enzyme action. Why might formation of the ES complex be important for providing specificity? As illustrated in Figure 1-1 above, binding of the enzyme to the substrate has a lock and key type interaction, meaning enzymes will only bind chemical species that fit the “lock”; the specific nature of this interaction helps ensure that only the appropriate substrate is acted on by the-enzyme. Once the ES complex is formed, the enzyme alters the structural conformation of the substrate in such a way that the substrate is converted to a product. The enzyme is simply used to facilitate the conversion of the substrate to product. Once this occurs, the enzyme is released and free to combine with more substrate so that more products can be generated.
RUBRIC |
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Excellent Quality 95-100%
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Introduction
45-41 points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned. |
Literature Support 91-84 points The background and significance of the problem and a clear statement of the research purpose is provided. The search history is mentioned. |
Methodology 58-53 points Content is well-organized with headings for each slide and bulleted lists to group related material as needed. Use of font, color, graphics, effects, etc. to enhance readability and presentation content is excellent. Length requirements of 10 slides/pages or less is met. |
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Average Score 50-85% |
40-38 points More depth/detail for the background and significance is needed, or the research detail is not clear. No search history information is provided. |
83-76 points Review of relevant theoretical literature is evident, but there is little integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are included. Summary of information presented is included. Conclusion may not contain a biblical integration. |
52-49 points Content is somewhat organized, but no structure is apparent. The use of font, color, graphics, effects, etc. is occasionally detracting to the presentation content. Length requirements may not be met. |
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Poor Quality 0-45% |
37-1 points The background and/or significance are missing. No search history information is provided. |
75-1 points Review of relevant theoretical literature is evident, but there is no integration of studies into concepts related to problem. Review is partially focused and organized. Supporting and opposing research are not included in the summary of information presented. Conclusion does not contain a biblical integration. |
48-1 points There is no clear or logical organizational structure. No logical sequence is apparent. The use of font, color, graphics, effects etc. is often detracting to the presentation content. Length requirements may not be met |
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